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human foreskin fibroblasts hff  (ATCC)


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    Structured Review

    ATCC human foreskin fibroblasts hff
    Human Foreskin Fibroblasts Hff, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+foreskin+fibroblasts+hff/HFF-1/pmc13092094-59-11-17
    Average 99 stars, based on 1570 article reviews
    human foreskin fibroblasts hff - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Screening of purine nucleoside analogues against intracellular Toxoplasma gondii.
    Article Snippet: .. All T. gondii tachyzoites used in this project were cultured in human foreskin fibroblasts (HFF), sourced from ATCC (SCRC-1041). ..

    Article Title: Identification of TgENT1 as the TgUUT1 Uracil/Uridine Transporter of Toxoplasma gondii
    Article Snippet: .. T. gondii tachyzoites of the RH and mutant lines were cultured in human foreskin fibroblasts (HFF), sourced from ATCC (SCRC-1041). .. Parasites were passaged routinely as a confluent monolayer of HFF in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4.5 gL −1 glucose (Sigma, St Louis, MO, USA), supplemented with 10% ( v / v ) fetal bovine serum (Gibco, Life Technologies, Paisley, UK), 4 mM L-glutamine and penicillin/streptomycin (Life Technologies) antibiotics and grown at 37 °C with 5% CO 2 .

    Article Title: Screening of purine nucleoside analogues against intracellular Toxoplasma gondii
    Article Snippet: .. All T. gondii tachyzoites used in this project were cultured in human foreskin fibroblasts (HFF), sourced from ATCC (SCRC-1041). ..

    Article Title: Screening of purine nucleoside analogues against intracellular Toxoplasma gondii
    Article Snippet: .. All T. gondii tachyzoites used in this project were cultured in human foreskin fibroblasts (HFF), sourced from ATCC (SCRC-1041). ..

    Mutagenesis:

    Article Title: Identification of TgENT1 as the TgUUT1 Uracil/Uridine Transporter of Toxoplasma gondii
    Article Snippet: .. T. gondii tachyzoites of the RH and mutant lines were cultured in human foreskin fibroblasts (HFF), sourced from ATCC (SCRC-1041). .. Parasites were passaged routinely as a confluent monolayer of HFF in Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4.5 gL −1 glucose (Sigma, St Louis, MO, USA), supplemented with 10% ( v / v ) fetal bovine serum (Gibco, Life Technologies, Paisley, UK), 4 mM L-glutamine and penicillin/streptomycin (Life Technologies) antibiotics and grown at 37 °C with 5% CO 2 .



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    ATCC human foreskin fibroblast cell line hff
    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
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    ATCC human foreskin fibroblast hff cells
    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell <t>line</t> <t>HFF-1</t> as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)
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    Average 96 stars, based on 1 article reviews
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    ATCC human foreskin fibroblast hff 1
    Cytotoxicity assay of ZnFe 2 O 4 /HNT formulations <t>treated</t> <t>HFF-1</t> cells. Cell viability (%) of HeLa cells following treatment with nanocarrier formulations for ( a ) 24 h, ( b ) 48 h, and ( c ) 72 h, as determined by the MTT assay. Cells were exposed to increasing concentrations of the indicated formulations, including ZnFe 2 O 4 /HNT, ZnFe 2 O 4 /HNT/Cp, ZnFe 2 O 4 /HNT/Cp/FA/PEG, ZnFe 2 O 4 /HNT/Cbpt, Cbpt, and Cp. Statistical significance is indicated as ns: not significant,* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.
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    Image Search Results


    Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Journal: BMC Cancer

    Article Title: Targeting EpCAM expression via near-infrared fluorescent antibodies enables microscopic delineation of primary and recurrent HNSCC

    doi: 10.1186/s12885-026-16172-2

    Figure Lengend Snippet: Expression of EpCAM on cell lines of SCC-UADT, HNSCC-associated fibroblasts, and normal fibroblasts. A Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 (ESCC) and FaDu (HNSCC), the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody VU1D9 (high EpCAM-affinity) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu). B Representative histogram and quantitative data on the percentage of EpCAM-positive cells as well as on the expression level of EpCAM of the SCC-UADT cell lines KYSE-30 and FaDu, the HNSCC-associated fibroblast cell line CAF-4, and the normal fibroblast cell line HFF-1 as assessed by flow cytometry upon immunostaining with the AlexaFluor488-labeled anti-EpCAM antibody MT201 (intermediate EpCAM-affinity; clinically validated) (mean ± SEM for n = 4; * p < .05 vs. CAF; # p < .05 vs. HFF; § p < .05 vs. KYSE-30; & p < .05 vs. FaDu)

    Article Snippet: Human foreskin fibroblast cell line HFF-1 was purchased from ATCC (Manassas, USA; SCRC-1041).

    Techniques: Expressing, Flow Cytometry, Immunostaining, Labeling

    Cytotoxicity assay of ZnFe 2 O 4 /HNT formulations treated HFF-1 cells. Cell viability (%) of HeLa cells following treatment with nanocarrier formulations for ( a ) 24 h, ( b ) 48 h, and ( c ) 72 h, as determined by the MTT assay. Cells were exposed to increasing concentrations of the indicated formulations, including ZnFe 2 O 4 /HNT, ZnFe 2 O 4 /HNT/Cp, ZnFe 2 O 4 /HNT/Cp/FA/PEG, ZnFe 2 O 4 /HNT/Cbpt, Cbpt, and Cp. Statistical significance is indicated as ns: not significant,* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Zinc Ferrite-Integrated Halloysite Nanotubes as a Platform for Folate-Mediated Targeted Cisplatin Delivery

    doi: 10.3390/ijms27104284

    Figure Lengend Snippet: Cytotoxicity assay of ZnFe 2 O 4 /HNT formulations treated HFF-1 cells. Cell viability (%) of HeLa cells following treatment with nanocarrier formulations for ( a ) 24 h, ( b ) 48 h, and ( c ) 72 h, as determined by the MTT assay. Cells were exposed to increasing concentrations of the indicated formulations, including ZnFe 2 O 4 /HNT, ZnFe 2 O 4 /HNT/Cp, ZnFe 2 O 4 /HNT/Cp/FA/PEG, ZnFe 2 O 4 /HNT/Cbpt, Cbpt, and Cp. Statistical significance is indicated as ns: not significant,* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.

    Article Snippet: The cervical cancer cell line HeLa (ATCC ® CCL-2TM, ATCC, Manassas, VA, USA) and the human foreskin fibroblast HFF-1 (ATCC ® SCR-C-1041TM, ATCC, Manassas, VA, USA) were purchased from the American Type Culture Collection (ATCC).

    Techniques: Cytotoxicity Assay, MTT Assay